Rabies Virus (RABV)

Rabies Virus (RABV) is a type of enveloped, bullet-shaped, negative-single stranded RNA virus in the genus of Lyssavirus of the family Rhabdoviridae.The RNA genome of the virus is approximately 12 kb and encodes five viral proteins which includes nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G) and the viral RNA polymerase (L) from 3 ' end to 5' end.

Through reverse genetics, recombinant RABV constructed by Wickersham et al. is based on SAD-B19 vaccine strains. The glycoprotein gene of the modified RABV was completely removed (G-deleted) that it lose the ability to across synapses, but its replication and transcription is not affected. The RABV-∆G can be used to reverse high brightness fine marking neurons. If we provide the G protein first (by first injection with AAV-G ), the recombinant RABV will have the ability to spread retrograde across the synaptic.

To further refine the targeting of G-deleted RABV to specific starter cells, this virus was modified by combining with the envelope proteins EnvA of Avian Sarcoma and Leukosis Virus (ASLV). The infectivity of these viruses is restricted to cells carrying the TVA receptor. Of course, the receptor TVA and the EnvA protein must first be provided.
After years of research, BrainVTA has established a stable production platform, and could offer rabies virus (RABV) services to promote RABV-based neuronal tracing studies.

Applications
● Changes in neural networks during neural development
● Monosynaptic tracing in specific type of neuron
Popular RABV particles

Cat#	Name	Description
R01002	RV-EnVA-△G-dsRed	Combined with AAV-TVA and AAV-RVG, Retrograde,Monosynaptic
R01003	RV-EnVA-△G-GCaMP6s-dsRed	Combined with AAV-TVA and AAV-RVG, Retrograde,Monosynaptic, Calcium signal detection
R01004	RV-EnVA-△G-mCherry	Combined with AAV-TVA and AAV-RVG, Retrograde,Monosynaptic
R01010	RV-EnVA-△G-eGFP-synphRFP	Combined with AAV-TVA and AAV-RVG, Retrograde,Monosynaptic
R01012	RV-EnVA-△G-ChR2-dsRed	Combined with AAV-TVA and AAV-RVG, Retrograde,Monosynaptic
R01013	RV-EnVA-△G-pre-mGRASP-dsRed	Combined with AAV-TVA and AAV-RVG, Retrograde,Monosynaptic
R01014	RV-EnVA-△G-post-mGRASP-dsRed	Combined with AAV-TVA and AAV-RVG, Retrograde,Monosynaptic
R03001	RV-N2C(G)-△G-eGFP	Combined with AAV-RVG, Retrograde,Monosynaptic
R03002	RV-N2C(G)-△G-dsRed	Combined with AAV-RVG, Retrograde,Monosynaptic
R03010	RV-N2C(G)-△G-EGFP-synphRFP	Combined with AAV-RVG, Retrograde,Monosynaptic

References
1. Etessami R, Conzelmann KK, Fadai-Ghotbi B, Natelson B, Tsiang H, Ceccaldi PE. Spread and pathogenic characteristics of a G-deficient rabies virus recombinant: an in vitro and in vivo study. J Gen Virol. 2000 Sep;81(Pt 9):2147-53.
2. Osakada F, Callaway EM. Design and generation of recombinant rabies virus vectors. Nat Protoc. 2013 Aug;8(8):1583-601.
3. Callaway EM, Luo L. Monosynaptic Circuit Tracing with Glycoprotein-Deleted Rabies Viruses. J Neurosci. 2015 Jun 17;35(24):8979-85.

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