COVID-19 Coronavirus Research Tools
Pre-Made AAVs Center
Neuronal Tracing Tool


Construction and Packaging Service of virus Vector

Construction and Packaging Services of AAV

Large scale AAV production

Lentivirus (LVs)
Lentivirus (LVs) are single stranded RNA (sRNA) viruses, which are a subgroup of the retrovirus family. They can integrate into the host genome in both dividing and non-dividing cells, with a wide range of cell, resulting in long-term expression both in vitro and in vivo. The delivery vehicles based on HIV-1 have been developed by BrainVTA.

The advantages of LV compared to other viral vectors
● Long-term gene expression: Lentivirus can achieve long-term stable expression of target genes by integrating genes into the host cell genome, and will not be lost with cell division, which is the first choice for cell experiments.
● High safety. No pathogenicity has been found, and it has been used in CAR-T therapy in human;
● Low immunogenicity. Direct injection of tissues in vivo is not easy to cause an immune response and is suitable for animal experiments.

Viral vector AAV LV Ad
Genome ssDNA ssRNA (+) dsDNA
Coat Naked Enveloped Naked
Type Non-integrating Integrating Non-integrating
Infection Dividing and non-dividing cells Dividing and non-dividing cells Dividing and non-dividing cells
4.7kb 6kb 7.5kb
Potentially long-lasting Long-lasting Transient
Very Low Low High
Titer Up to 1012-13v.g/ml Up to 109TU/ml Up to 1012pfu/ml
Expression abundance High-level expression Moderate to high level expression High-level expression
Safety No pathogenicity has been found yet,
Has been approved by the EU and FDA,
Used as a carrier for gene therapy drugs
No pathogenicity has been found yet,
It has been used in CAR-T therapy in the human
May cause some coughing and runny nose

Packaging service
Step1: Viral vector preparation. Insert the target gene fragment into the lentiviral vector.
Step2: Co-infection in 293T cells. lentiviral plasmid and helper plasmid (Helper1.0 and Helper2.0)
Step3: Virus collection. The cell supernatant is collected by centrifugation after 48-72h of transfection.
Step4: Transformation and concentration. Process the cell supernatant by 0.45um filtration and ultracentrifugation.
Step5: Quality testing. The commonly used methods for titer testing are fluorescence, drug screening and absolute quantitative qPCR.

How do I obtain virus packaging services?
● You can click Pre-Made LVs to find out whether the list contains what you need.
● Also, You can obtain custom-Made LVs service by clicking here.

Characteristics of (HIV) Based Vectors 
● Infects dividing and non-dividing cells;
● Long term, stable gene expression resulting from genomic integration;
● Pseudotyping with a VSV-G envelope glycoproteins that broaden cell tropism;
● Factors such as expression cassette size or choice of envelope will influence titer;
● HIV accommodates insert sizes of 6.5 kb. Larger expression cassettes can be packaged but decreases in titers will be expected.

Selection of vectors
BrainVTA provides a complete lentivirus product system, which can manipulate coding and non-coding genes, such as lncRNA, microRNA, and circRNA. For more details, please contact [email protected]

Ready to order?
Further information about the lentiviral vectors, just click the Pre-Made LVs  or the Custom-Made LVs Button and submit your needs to us. 

50% discount for Pre-Made AAVs