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BrainVTA

Construction and Packaging Service of virus Vector

Construction and Packaging Services of AAV

Large scale AAV production

Lentivirus (LVs)
Lentivirus (LVs) are single stranded RNA (sRNA) viruses, which are a subgroup of the retrovirus family. They can integrate into the host genome in both dividing and non-dividing cells, with a wide range of cell, resulting in long-term expression both in vitro and in vivo. The delivery vehicles based on HIV-1 have been developed by BrainVTA.

The advantages of LV compared to other viral vectors
● Long-term gene expression: Lentivirus can achieve long-term stable expression of target genes by integrating genes into the host cell genome, and will not be lost with cell division, which is the first choice for cell experiments.
● High safety. No pathogenicity has been found, and it has been used in CAR-T therapy in human;
● Low immunogenicity. Direct injection of tissues in vivo is not easy to cause an immune response and is suitable for animal experiments.

Viral vector AAV LV Ad
Genome ssDNA ssRNA (+) dsDNA
Coat Naked Enveloped Naked
Type Non-integrating Integrating Non-integrating
Infection Dividing and non-dividing cells Dividing and non-dividing cells Dividing and non-dividing cells
Packaging
Capacity
4.7kb 6kb 7.5kb
Transgene
expression
Potentially long-lasting Long-lasting Transient
Immune
Response
Very Low Low High
Titer Up to 1012-13v.g/ml Up to 109TU/ml Up to 1012pfu/ml
Expression abundance High-level expression Moderate to high level expression High-level expression
Safety No pathogenicity has been found yet,
Has been approved by the EU and FDA,
Used as a carrier for gene therapy drugs
No pathogenicity has been found yet,
It has been used in CAR-T therapy in the human
May cause some coughing and runny nose

Packaging service
Step1: Viral vector preparation. Insert the target gene fragment into the lentiviral vector.
Step2: Co-infection in 293T cells. lentiviral plasmid and helper plasmid (Helper1.0 and Helper2.0)
Step3: Virus collection. The cell supernatant is collected by centrifugation after 48-72h of transfection.
Step4: Transformation and concentration. Process the cell supernatant by 0.45um filtration and ultracentrifugation.
Step5: Quality testing. The commonly used methods for titer testing are fluorescence, drug screening and absolute quantitative qPCR.

How do I obtain virus packaging services?
● You can click Pre-Made LVs to find out whether the list contains what you need.
● Also, You can obtain custom-Made LVs service by clicking here.

Characteristics of (HIV) Based Vectors 
● Infects dividing and non-dividing cells;
● Long term, stable gene expression resulting from genomic integration;
● Pseudotyping with a VSV-G envelope glycoproteins that broaden cell tropism;
● Factors such as expression cassette size or choice of envelope will influence titer;
● HIV accommodates insert sizes of 6.5 kb. Larger expression cassettes can be packaged but decreases in titers will be expected.

Selection of vectors
BrainVTA provides a complete lentivirus product system, which can manipulate coding and non-coding genes, such as lncRNA, microRNA, and circRNA. For more details, please contact [email protected]

Ready to order?
Further information about the lentiviral vectors, just click the Pre-Made LVs  or the Custom-Made LVs Button and submit your needs to us. 




50% discount for Pre-Made AAVs