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BrainVTA

Construction and Packaging Service of virus Vector

Construction and Packaging Services of AAV

Large scale AAV production

Genome Editing Service
Gene editing is the insertion, deletion or replacement of DNA at a specific site in the genome of an organism or cell. There are the Zinc finger nucleases (ZFNs), transcription-activator like effector nucleases (TALEN), meganucleases and the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) system have been used for genome editing. CRISPR/Cas9 is simpler, faster, cheaper, and more accurate than other genome editing methods, therefore many scientists who perform genome editing now use CRISPR.

                     
                Transcriptional activation                                                                                    Knock-in 
BrainVTA is a professional biotechnology company in CRISPR editing field. BrainVTA could offer a range of gene editing services, which is including but not limiting to gene knockout,  gene knock-in, transcriptional activation, site-directed mutation. Such genome editing services can offer researchers the comprehensive workflows--from experimental design, to cell line development and validation.
sgRNA screening 
 
Screening method Advantage  Disadvantage
Cas9 protein in vitro  cleavage  assay   No plasmid required,  short cycle     In vitro screening does not reflect the real state of the genome.
T7 endonuclease I assay Closer to the real reaction Cell is needed.
Markers and Reporters
Vectors with mCherry or eGFP reporter genes for monitoring transfection or transduction efficiencies. Stable cell are selected with puromycin marker.
Whole Delivery Formats
● Individual sgRNA or Donor constructs of 5 µg purified plasmid.
● sgRNA screening report.
Lentivirus or AAV virus.
Key informations
● Fast and high efficiency
● Strong background in CRISPR editing field
● Greater gene-editing efficiency 
● Capable of editing multiple genes simultaneously
● The most competitive price

If you have any special requirements in genome editing service, just email us at [email protected] or click the Send Request button.

Reference:
[1] Eric Murillo-Rodríguez, Rocha N B , André Barciela Veras, et al. The End of Snoring? Application of CRISPR/Cas9 Genome Editing for Sleep Disorders[J]. Sleep and Vigilance, 2018, 2(1):13-21.
[2] Yao, X., Wang, X., Hu, X., Liu, Z., Liu, J., Zhou, H., Shen, X., Wei, Y., Huang, Z., Ying, W., et al. (2017). Homology-mediated end joining-based targeted integration using CRISPR/Cas9. Cell research 27, 801-814.
[3] Wiles M V , Qin W , Cheng A W , et al. CRISPR–Cas9-mediated genome editing and guide RNA design[J]. Mammalian Genome, 2015, 26(9-10).
[4] Wang H , Yang H , Shivalila C , et al. One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering[J]. Cell, 2013, 153(4):910-918.

 

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