COVID-19 Coronavirus Research Tools
Pre-Made AAVs Center
Neuronal Tracing Tool

BrainVTA

Construction and Packaging Service of virus Vector

Construction and Packaging Services of AAV

Large scale AAV production

Specific HypothalamicNeurons Required for Sensing Conspecifi
AAVs encoding Cre-inducible avian retroviral receptor (TVA)-GFP and rabies glycoprotein (RG) were used for mono-synaptic retrograde tracing. (From BrainVTA)
The viruses used in this article from BrainVTA are in the table below
Tracing Helper  PT-0023 AAV2/9-EF1a-DIO-RVG
 PT-0021 AAV2/9-EF1a-DIO-His-EGFP-2A-TVA
RV  R01002 RV-EnVA-DG-DsRed
 R01003 RV-EnVA-DG-GCaMP6s-DsRed
Ai-Xiao Chen, Jing-Jing Yan, Wen Zhang, Lei Wang, Zi-Xian Yu, Xiao-Jing Ding, Dan-Yang Wang, Min Zhang, Yan-Li Zhang, Nan Song, Zhuo-Lei Jiao, Chun Xu, Shu-Jia Zhu, Xiao-Hong Xu
Pub Date: 2020-09-21, DOI: 10.1016/j.neuron.2020.08.025, Email: [email protected]
The hypothalamus regulates innate social interactions, but how hypothalamic neurons transduce sex-related sensory signals emitted by conspecifics to trigger appropriate behaviors remains unclear. Here, we addressed this issue by identifying specific hypothalamic neurons required for sensing conspecific male cues relevant to inter-male aggression. By in vivo recording of neuronal activities in behaving mice, we showed that neurons expressing dopamine transporter (DAT+) in the ventral premammillary nucleus (PMv) of the hypothalamus responded to male urine cues in a vomeronasal organ (VNO)-dependent manner in naive males. Retrograde trans-synaptic tracing further revealed a specific group of neurons in the bed nucleus of the stria terminalis (BNST) that convey male-relevant signals from VNO to PMv. Inhibition of PMvDAT+ neurons abolished the preference for male urine cues and reduced inter-male attacks, while activation of these neurons promoted urine marking and aggression. Thus, PMvDAT+ neurons exemplify a hypothalamic node that transforms sex-related chemo-signals into recognition and behaviors.

Figure 1. PMvDAT+ Neurons Receive Direct Synaptic Inputs from v-BNST.
This study is aimed to explore how hypothalamic neurons transduce sex-related sensory signals emitted by conspecifics to trigger innate social interactions. The authors began their study by identifying specific hypothalamic neurons required for sensing conspecific male cues relevant to inter-male aggression. The results highlight the essential role for PMvDAT+ neurons in relaying conspecific male cues to trigger appropriate behaviors.
 
BrainVTA offers viral vector construction & virus packaging services for AAV, LV, RABV, PRV, HSV and VSV that help researchers explore questions about genes, neurons, circuitry structure, function of brain network, mechanism and treatment of diseases.
If you have any needs, just email us at [email protected].


 

50% discount for Pre-Made AAVs