Distinct Characteristics of Odor-evoked Calcium and Electro-p

AAV-GCaMp6s was used to simultaneously record odor-evoked Ca²⁺ signals and electrophysiological signals from M/Ts. (From BrainVTA)

The viruses used in this article from BrainVTA are in the table below

Calcium sensors

PT-0071 rAAV-EF1α-DIO-GCaMp6s-WPRE-hGH polyA

Han Xu, Chi Geng, Xinzhong Hua, Penglai Liu, Jinshan Xu, Anan Li
Pub Date: 2021-04-15, DOI: 10.1007/s12264-021-00680-1, Email: [email protected]
Fiber photometry is a recently-developed method that indirectly measures neural activity by monitoring Ca²⁺ signals in genetically-identiﬁed neuronal populations. Although ﬁber photometry is widely used in neuroscience research, the relationship between the recorded Ca²⁺ signals and direct electrophysiological measurements of neural activity remains elusive. Here, we simultaneously recorded odor-evoked Ca²⁺ and electrophysiological signals [single-unit spikes and local ﬁeld potentials (LFPs)] from mitral/tufted cells in the olfactory bulb of awake, head-ﬁxed mice. Odors evoked responses in all types of signal but the response characteristics (e.g., type of response and time course) differed. The Ca²⁺ signal was correlated most closely with power in the b-band of the LFP. The Ca²⁺ signal performed slightly better at odor classiﬁcation than high-c oscillations, worse than single-unit spikes, and similarly to b oscillations. These results provide new information to help researchers select an appropriate method for monitoring neural activity under speciﬁc conditions.

Figure 1. Simultaneous recording of odor-evoked spikes, LFPs, and population Ca²⁺ signals from M/Ts.

The study is aimed to explore the relationship between the recorded Ca²⁺ signals and direct electrophysiological measurements of neural activity. In this study, the authors used a mouse model with M/T-speciﬁc GCaMP6s expression to simultaneously record odor-evoked Ca²⁺ signals and electrophysiological signals from M/Ts in awake, head-ﬁxed mice. The ﬁndings show how Ca²⁺ signals recorded by ﬁber photometry relate to electrophysiological recordings and have implications for the application of ﬁber photometry throughout the brain.

BrainVTA offers viral vector construction & virus packaging services for AAV, LV, RABV, PRV, HSV and VSV that help researchers explore questions about genes, neurons, circuitry structure, function of brain network, mechanism and treatment of diseases.
If you have any needs, just email us at [email protected].