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Protocol for intravitreal injection
Intravitreous injection is a widely used technique in visual sciences research. It can be used to establish animal models with ocular diseases or as direct application of local treatment. We present here a protocol that uses a intravitreous injection with a viral vector in the mouse. The experimental process of intravitreous injection is as follows.
Before surgery, mouse is anesthetized by intraperitoneal injection of 2 % sodium pentobarbital (50 mg/kg). Wait until the mouse becomes motionless (~5 min).
Apply eye ointment such as Oculentum simplex, Teva Pharmachemie to the mouse eyes as to keep eyes moist.
Position the mouse and expose the superior nasal region of the eye, then use a 30-gauge needle to puncture the superior nasal sclera at the level of the par plana and gently inject the virus. when finishing injection, keep the needle in place for about a few seconds, then gently remove it. In general, the injection volume is 1~3 μl/eye. Please note that avoid touching the ocular muscle and vessels.
Generally, each mouse is injected with only one eye, and the other one serves as a control. Topical antibacterial treatment on virus-injected eyes is necessary to prevent infection.
After one week, use a fundus camera for fundus examination.

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