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Dorsal raphe nucleus to anterior cingulate cortex 5-HTergic n
AAV-ChR2 and AAV-eNpHR3.0 were used for optogenetic manipulation. AAV-5HTsensor 2.1 was used to detecte the dynamics of 5-HT within the ACC. AAV-DIO-GCaMp6s was used for fiber photometry experiment. AAV-hM4Di(Gi) and AAV-hM3Dq(Gq) were used for chemogenetics manipulation. (From BrainVTA)
The viruses used in this article from BrainVTA are in the table below
Optogenetic  PT-0002 rAAV-Ef1a-DIO-ChR2-mCherry-WPRE-pA
 PT-0007 rAAV-Ef1a-DIO-eNpHR3.0-mCherry-WPRE-pA
Neurotransmitter sensors  PT-1826 rAAV-hSyn-5HT2.1-WPRE-hGHpA
Calcium sensors  PT-0071 rAAV-EF1a-DIO-GCaMp6s-WPRE-hGH pA
Chemogenetics  PT-0043 rAAV-Ef1a-DIO-hM4Di(Gi)-mCherry-WPRE-pA
 PT-0042 rAAV-Ef1a-DIO-hM3Dq(Gq)-mCherry-WPRE-pA
CRE Recombinase  PT-0396 rAAV(Retro)-TPH2-Cre-WPRE-pA
Control  PT-0013 rAAV-Ef1a-DIO-mCherry-WPRE-pA
 PT-0241 rAAV-hSyn-EGFP-WPRE-hGH-pA
Laifu Li, Li-Zi Zhang, Zhi-Xiong He, Huan Ma, Yu-Ting Zhang, Yu-Feng Xun, Wei Yuan, Wen-Juan Hou, Yi-Tong Li, Zi-Jian Lv, Rui Jia, Fa-Dao Tai
Pub Date: 2021-06-03, DOI: 10.7554/elife.67638, Email: [email protected]
Consolation is a common response to the distress of others in humans and some social animals, but the neural mechanisms underlying this behavior are not well characterized. By using socially monogamous mandarin voles, we found that optogenetic or chemogenetic inhibition of 5-HTergic neurons in the dorsal raphe nucleus (DR) or optogenetic inhibition of 5-HT terminals in the anterior cingulate cortex (ACC) significantly decreased allogrooming time in the consolation test and reduced sociability in the three-chamber test. The release of 5-HT within the ACC and the activity of DR neurons were significantly increased during allogrooming, sniffing and social approaching. Finally, we found that the activation of 5-HT1A receptors in the ACC was sufficient to reverse consolation and sociability deficits induced by the chemogenetic inhibition of 5-HTergic neurons in the DR. Our study provided first direct evidence that DR-ACC 5-HTergic neural circuit is implicated in consolation-like behaviors and sociability.

Figure 1. Chemogenetic modulation of DR 5-HT neuron activities in the DR-ACC neural circuit.
In the present study, the authors have demonstrated a crucial role for the DR→ACC 5-HTergic neural circuit in the regulation of consolation-like behaviors and sociability in mandarin voles for the first time.
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