AAV-GCaMP6m was used to determine the effects of chronic social isolation on the neuronal activity in the NAcc shell. (From
BrainVTA)
The viruses used in this article from BrainVTA are in the table below
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Calcium sensors |
PT-0148 rAAV-hSyn-GCaMP6m-WPRE-pA |
Xueni Zhang, Yufeng Xun, Limin Wang, Jing Zhang, Wenjuan Hou, Huan Ma, Wenqi Cai, Laifu Li, Qianqian Guo, Yitong Li, Zijian Lv, Rui Jia, Fadao Tai, Zhixiong He
Pub Date: 2021-04-21,
DOI: 10.1016/j.brainres.2021.147497,
Email: [email protected]
In the early stage of life, experiencing social isolation can generate long-lasting deleterious effects on behaviors and brain development. However, the effects of chronic social isolation during adolescence on social behaviors and its underlying neurobiological mechanisms remain unclear. The present study found that four weeks of social isolation during adolescence impaired social recognition ability in the three-chamber test and five-trial social recognition test, and increased aggressive-like behaviors, but reduced environmental exploration, as showed in the social interaction test. Chronic social isolation decreased levels of dopamine D2 receptor in the shell of the nucleus accumbens (NAcc) and medial prefrontal cortex. It also reduced TH in the NAcc. Using in vivo fiber photometry, it was also found that isolated mice displayed a reduction in NAcc shell activity upon exploring unfamiliar social stimuli. An injection of a 100 ng dose of the D2R agonist quinpirole into the shell of the NAcc reversed behavioral abnormalities induced by chronic social isolation. These data suggest that the dopamine system is involved in alterations in social behaviors induced by chronic social isolation. This finding sheds light on the mechanism underlying abnormalities in social behavior induced by adolescent chronic social isolation and provides a promising target to treat mental diseases relevant to social isolation.
Figure 1. Image showing GCaMP6m expression in the NAcc shell, with optic fiber placement indicated.
In this study, the authors employed multiple experiments to investigate the involvement of dopamine in the effects of chronic social isolation on social behaviors. This study can shed light on the underlying brain mechanism of social dysfunction induced by social isolation.
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