AAV tracing helper and RV were used for cell-type-specific retrograde tracing to identify the upstreaminput of ARC
AgRP neurons. AAV -hM4D(Gi) was used for chemogenetics manipulation (From
BrainVTA)
The viruses used in this article from BrainVTA are in the table below
|
Chemogenetics |
PT-0043-AAV2/9-Ef1a-DIO- hM4D(Gi)-mCherry-WPRE-pA |
|
AAV tracing helper |
PT-0021-AAV2/9-Ef1a-DIO-His-EGFP-2A-TVA-WPRE-pA
PT-0023-AAV2/9-Ef1a-DIO-RVG-WPRE-pA |
|
Control |
PT-0013-AAV2/9-Ef1a-DIO-mCherry-WPRE-pA |
|
RV |
R01002-RV-EnvA-ΔG-dsRed |
Shuo Yang, Yu Lin Tan, Xiaohua Wu, Jingjie Wang, Jingjing Sun, Anqi Liu, Linhua Gan, Bo Shen, Xiaocui Zhang, Yu Fu, Ju Huang
Pub Date: 2021-08-10,
DOI: 10.1016/j.celrep.2021.109502,
Email: [email protected]
Enhanced appetite occurs as a means of behavioral thermoregulation at low temperature. Neural circuitry mediating this crosstalk between behavioral thermoregulation and energy homeostasis remains to be elucidated. We find that the hypothalamic orexigenic agouti-related neuropeptide (AgRP) neurons in the arcuate nucleus (ARC) are profoundly activated by cold exposure. The calcium signals in ARCAgRP neurons display an immediate-response pattern in response to cold stimulation. Cold-responsive neurons in the medial preoptic area (mPOA) make excitatory synapses onto ARCAgRP neurons. Inhibition of either ARCAgRP neurons or ARC-projecting mPOA neurons attenuates cold-evoked feeding, while activation of the mPOA-to-ARC projection increases food intake. These findings reveal an mPOA-ARCAgRP neural pathway that modulates cold-evoked feeding behavior.
In this study, the authors reveal that ARC
AgRP neurons are profoundly activated in cold exposure and discover an mPOA-ARC
AgRP neural pathway that regulates cold-evoked feeding behavior. This study furthers our understanding of the neural circuitry that bridges the connection between
behavioral thermoregulation and energy homeostasis.
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