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A circuit of mossy cells controls the efficacy of memory retr
AAV tracing helper and RV were used for monosynaptic tracing. (From BrainVTA)
The viruses used in this article from BrainVTA are in the table below
AAV tracing helper  AAV-EF1a-DIO-TVA/G/GFP
RV  DRV (Sad B19 strain)
Xinyan Li, Wenting Chen, Quntao Yu, Qingping Zhang, Tongmei Zhang, Xian Huang, Hao Li, Aodi He, Hongyan Yu, Wei Jing, Huiyun Du, Xiao Ke, Bing Zhang, Qing Tian, Rong Liu, Youming Lu
Pub Date: 2021-02-16, DOI: 10.1016/j.celrep.2021.108741, Email: [email protected]
Mossy cells (MCs) are a unique group of excitatory neurons in the hippocampus, a brain region important for emotion, learning, and memory. Due to the lack of a reliable method to isolate MCs from other cell types, how MCs integrate neural information and convey it to their synaptic targets for engaging a specific function are still unknown. Here, we report that MCs control the efficacy of spatial memory retrieval by synapsing directly onto local somatostatin-expressing (SST) cells. MC-SST synaptic transmission undergoes long-term potentiation (LTP), requiring Gria2-lacking Ca2+-permeable anti-α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptor (Ca2+AR). A long noncoding RNA (Gria2I) is associated with Gria2 transcriptional repressors in SST cells. Silencing Gria2I induces Gria2 transcription, blocks LTP of MCs-SST synaptic transmission, and reduces the efficacy of memory retrieval. Thus, MCs directly and functionally innervate local SST neurons, and this innervation controls the efficacy of spatial memory retrieval by Gria2I inhibition of Gria2 transcription.
In the present study, the authors have uncovered that MCs decode the efficacy of spatial memory retrieval by directly and functionally innervating local SST neurons. They have identified a long, non-coding RNA, named Gria2I, associated with Gria2 transcriptional regulators and that controls synapticGria2-lacking/Ca2+ -permeable anti-a-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptor (Ca2+AR). Activation of Ca2+AR induces activity-dependent long-term potentiation (LTP) of MC-SST synaptic transmission for the effective retrieval of spatial memory.
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