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The NAergic locus coeruleus-ventrolateral preoptic area neura
AAV-hChR2 and AAV-eNpHR3.0 were used for optogenetic experiments. (From BrainVTA)
The viruses used in this article from BrainVTA are in the table below
 CRE Recombinase  PT-0781 mTH-NLS-Cre-rAAV2/9
Optogenetic  PT-0001 EF1a-DIO-hChR2(H134R)-eYFP-rAAV2/9
 PT-0003 EF1a-DIO-eNpHR3.0-eYFP-rAAV2/9
Yue Liang, Wu Shi, Anfeng Xiang, Dandan Hu, Liecheng Wang, Ling Zhang
Pub Date: 2021-07-15, DOI: 10.1016/j.cub.2021.06.031, Email: [email protected]
The locus coeruleus (LC), which is located in the brain stem, plays an important role in promoting arousal. However, the neural circuitry underlying this function remains unclear. Using cortical electroencephalography combined with optrode recording, we found that LC noradrenergic (LCNA) neurons exhibit high activity during wakefulness, while suppressing the activity of these neurons causes a reduction in wakefulness. Viral tracing showed that LCNA neurons directly project to the ventrolateral preoptic area (VLPO) and that optogenetic activation of the noradrenergic (NAergic) LC-VLPO (NAergicLC-VLPO) neural circuit promotes arousal. Optrode recordings in the VLPO revealed two functionally distinct neuronal populations that were stimulated in response to the optogenetic activation of LCNA neurons. Consistently, we identified two types of VLPO neurons that exhibited different responses to NAergic projections from the LC mediated by discrete adrenergic receptors. Together, our results demonstrate that the NAergicLC-VLPO neural circuit is a critical pathway for controlling wakefulness and that a synergistic effect is produced by inhibition of sleep-active neurons in the VLPO through α2 receptors and activation of wake-active neurons in the VLPO through α1 and β receptors.
To explore the neural circuitry of the locus coeruleus in promoting arousal. Using cortical electroencephalography, viral tracing combined with optrode recording, the present study mainly clarifies the function and mechanism of the NAergicLC-VLPO neural circuit in the regulation of the sleep-wake cycle.
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