AAV-hM3D(Gq) and AAV-hM4D(Gi) were used for chemogenetics experiments. A Cre-dependent AAV-Casp3 virus was used to selectively ablate the subpopulation of VLM CA (From
BrainVTA)
The viruses used in this article from BrainVTA are in the table below
|
Chemogenetics |
PT-0019 rAAV-hSyn-DIO-hM3D(Gq)-mCherry-WPRE-pA
PT-0020 rAAV-hSyn-DIO-hM4D(Gi)-mCherry-WPRE-pA |
|
Neuron Ablation |
PT-0206 rAAV-flex-taCasp3-TEVp-WPRE-pA; AAV2/9 |
|
Control |
PT-0013 rAAV-EF1α-DIO-mCherry-WPRE-pA |
Ze Zhang, Yehua Li, Xueyuan Lv, Linlin Zhao, Xiaodong Wang
Pub Date: 2021-07-13,
DOI: 10.1073/pnas.2103505118,
Email: [email protected]
It is known that tumor growth can be influenced by the nervous system. It is not known, however, if tumors communicate directly with the central nervous system (CNS) or if such interactions may impact tumor growth. Here, we report that ventrolateral medulla (VLM) catecholaminergic (CA) neurons in the mouse brain are activated in tumor-bearing mice and the activity of these neurons significantly alter tumor growth in multiple syngeneic and spontaneous mouse tumor models. Specific ablation of VLM CA neurons by a dopamine-β-hydroxylase (DBH) promotor-activated apoptosis-promoting caspase-3 in Dbh-Cre mice as well as inhibition of these neurons by a chemogenetic method slowed tumor progression. Consistently, chemogenetic activation of VLM CA neurons promoted tumor growth. The tumor inhibition effect of VLM CA neuron ablation is mitigated in Dbh-Cre;Rag1−/− mice, indicating that this regulatory effect is mediated by the adaptive immune system. Specific depletion of CD8+ T cells using an anti-CD8+ antibody also mitigated the tumor suppression resulting from the VLM CA neuron ablation. Finally, we showed that the VLM CA neuronal ablation had an additive antitumor effect with paclitaxel treatment. Collectively, our study uncovered the role of VLM CA neurons in the mouse brain in controlling tumor growth in the mouse body.
In this study, the authors have discovered that the ventrolateral medulla (VLM) catecholaminergic (CA) neurons, a group of neurons that control mouse stress response, are activated in tumor-bearing mice, and the neuronal activity promotes tumor growth in multiple syngeneic and spontaneous mouse tumor models. The tumor-promoting effect of these VLM CA neurons is mediated by cytotoxic T cells. These findings establish an interaction between a tumor and a group of neurons in the mouse brain that influences tumorigenesis and tumor growth by modulating adaptive immunity.
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