AAV-hChR2 and AAV-eNpHR were used for optogenetic manipulation. (From
BrainVTA)
The viruses used in this article from BrainVTA are in the table below
|
Optogenetic |
PT-0297 rAAV-CaMKIIα-hChR2(H134R)-mCherry-WPRE-pA
PT-0724 rAAV-hSyn-eNpHR-YFP-WPRE-pA
PT-0003 rAAV-Ef1α-Dio-eNpHR-EYFP-WPRE-pA |
|
Control |
PT-0102 rAAV2/9-CaMKIIα-EYFP-WPRE-pA
PT-0012 rAAV-Ef1α-Dio-EYFP-WPRE-pA |
|
CRE Recombinase |
PT-0220 rAAV2/1-CaMKIIα-Cre-WPRE-pA |
Hui Zhang, Chunkui Zhang, Zhongwei Qu, Bing Li, Yujuan Su, Xia Li, Yan Gao, Yizheng Wang
Pub Date: 2021-06-09,
DOI: 10.1038/s41392-021-00545-z,
Email: [email protected]
Figure 1. STN–ANT plasticity is crucial for the motor control in Parkinson’s disease model.
In this study, the authors initially mapped the STN-projecting nuclei with a series of viral tracing studies, then investigated the role of enhanced neural activity in STN–ANT in motor control of PD model mice，next studied how the enhanced STN–ANT neural activity leads to chronic motor abnormalities in PD model mice. Together, the current findings provided at a comprehensive level (circuit, synaptic, and molecular) a clear dissection that synaptic plasticity regulated motor control.
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