RV viruses were used to validate the connectivity between hippocampal neurons and different types of neurons in the mPFC. AAV-hCHR2 and AAV- NpHR3.0 were used for optogenetics manipulation. AAV-GCaMP6s was used to explore how different types of GABAergic neurons in the mPFC respond to social behaviors. (All viruses were packaged by
BrainVTA)
The viruses used in this article from BrainVTA are in the table below
|
Optogenetic |
PT-0002 AAV2/9-EF1a-DIO-hCHR2(H134R)- mCherry
PT-0008 AAV2/9-CaMKII-NpHR3.0-YFP
PT-0007 AAV2/9-EF1a-DIO-NpHR3.0-mCherry |
|
Calcium sensors |
PT-0071 AAV2/9-EF1a-DIO-GCaMP6s |
|
CRE Recombinase |
PT0136 RetroAAV-Cre |
|
RV |
R01001 SAD-ΔG-GFP(EnvA)-RV
R01002 SAD-ΔG-DsRed(EnvA)-RV |
Qingtao Sun, Xiangning Li, Anan Li, Jianping Zhang, Zhangheng Ding, Hui Gong and Qingming Luo
Pub Date: 2020-02-11,
DOI: 10.1016/j.isci.2020.100894,
Email: [email protected]
Ventral hippocampus (vHIP) and medial prefrontal cortex (mPFC) are both critical regions for social behaviors. However, how their interactions affect social behavior is not well understood. By viral tracing, optogenetics, chemogenetics, and fiber photometry, we demonstrated that inhibition of vHIP or direct projections from vHIP to mPFC impaired social memory expression. Via rabies retrograde tracing, we found that all three major GABAergic neurons in mPFC received direct inputs from vHIP. Activation of parvalbumin positive (PV+) neurons in mPFC but not somatostatin positive (SST+) neurons can rescue the social memory impairment caused by vHIP inhibition. Furthermore, fiber photometry results demonstrated that social behaviors preferentially recruited PV+ neurons and inhibition of hippocampal neurons disrupted the activity of PV+ neurons during social interactions. These results revealed a new mechanism of how vHIP and mPFC regulate social behavior in complementarity with the existing neural circuitry mechanism.
Figure 1. Hippocampal Neurons Innervated Three Major GABAergic Neuron Types in the Mpfc
To reveal the connection between hippocampus-nucleus accumbens pathway and social behavior
, the authors employed viral tracing, optogenetics, chemogenetics, and fiber photometry to investigate the connectivity between vHIP and mPFC. The results showed that pyramidal neurons in vHIP can form direct monosynaptic connectivity with three major types of GABAergic neurons in mPFC and convey social memory to PV+mPFC neurons. These results provide a new mechanism of how vHIP-mPFC circuitry regulates social behavior, which can facilitate the study and treatment of social behavior deficits in neuropsychiatric disorders.
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