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Quantitative proteomics study of host response to virulent an
PRV443-EGFP and PRV152-EGFP were used to characterize the spread of PRV in mouse olfactory cortex.  (From BrainVTA
The viruses used in this article from BrainVTA are in the table below
 Pseudorabies virus  PRV443-EGFP
Hao-Long Zeng, Fei-Long Yu, Zhijian Zhang, Qing Yang, Sen Jin, Xiaobin He, Xi Chen, Ying Shen, Liming Cheng, Lin Guo, Fuqiang Xu
Pub Date: 2017-11-23,  DOI: 10.1016/j.bbapap.2017.11.010, Email:
Bartha, the pseudorabies virus (PRV) vaccine strain, is widely used in studies of neuronal circuit-tracing, due to its attenuated virulence and retrograde spreading. However, we know little regarding the molecular mechanisms of PRV infection and spreading between structurally connected neurons. In this study, we systematically analyzed the host brain proteomes after acute infection with PRV, attempting to identified the proteins involved in the processes. Mice were injected with PRV-Bartha and PRV-Becker (PRV-Bartha's wild-type parent strain) in the olfactory system, the proteomes of the brain and synaptosome were analyzed and compared at various infection intervals using mass spectrometry-based proteomics techniques. In all, we identified>100 PRV-infection regulated proteins at the whole-tissue level and the synaptosome level. While at whole-tissue level, bioinformatics analyses mapped most of the regulations to the inflammation pathways, at the synaptosome level, most of those to synaptic transmission, cargo transport and cytoskeleton organization. We established regulated protein networks demonstrating distinct cellular regulation pattern between the global and the synaptosome levels. Moreover, we identified a series of potentially PRV-strain-specific regulated proteins with diverse biological functions. This study may provide new clues for molecular mechanisms for PRV infection and spread.
Figure. 1. Trans-synaptic neuronal infection of PRV in mouse olfactory cortex.
In this study, the authors employed quantitative proteomics techniques to study host proteome profiles at both whole-tissue and synaptosome levels during PRV infection. This study may provide new clues for understanding the neural response of infection and spreading of the virus, and further to facilitate the designing of better PRV-based tracing systems.
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