The modified monosynaptic rabies tracing technique was used to achieve cell-type-specific monosynaptic tracing of extrinsic inputs. (From
BrainVTA)
The viruses used in this article from BrainVTA are in the table below
|
Tracing Helper |
PT-0021 rAAV-EF1α-DIO-His-EGFP-2a-TVA-WPRE-pA
PT-0023 rAAV-EF1α-DIO-RG-WPRE-pA |
|
RV |
R01001 SAD△G-DsRed-RV |
Chenggang Chen, Mingxiu Cheng, Tetsufumi Ito and Sen Song
Pub Date: 2018-02-26,
DOI: 10.1523/JNEUROSCI.2173-17.2018,
Email: [email protected]
The inferior colliculus (IC) is a critical integration center in the auditory pathway. However, because the inputs to the IC have typically been studied by the use of conventional anterograde and retrograde tracers, the neuronal organization and cell-type-specific connections in the IC are poorly understood. Here, we used monosynaptic rabies tracing and in situ hybridization combined with excitatory and inhibitory Cre transgenic mouse lines of both sexes to characterize the brainwide and cell-type-specific inputs to specific neuron types within the lemniscal IC core and nonlemniscal IC shell.We observed that both excitatory and inhibitory neurons of the IC shell predominantly received ascending inputs rather than descending or core inputs. Correlation and clustering analyses revealed two groups of excitatory neurons in the shell: one received inputs from a combination of ascending nuclei, and the other received inputs from a combination of descending nuclei, neuromodulatory nuclei, and the contralateral IC. In contrast, inhibitory neurons in the core received inputs fromthe same combination of all nuclei. After normalizing the extrinsic inputs, we found that core inhibitory neurons received a higher proportion of inhibitory inputs from the ventral nucleus of the lateral lemniscus than excitatory neurons. Furthermore, the inhibitory neurons preferentially received inhibitory inputs fromthe contralateral IC shell. Because IC inhibitory neurons innervate the thalamus and contralateral IC, the inhibitory inputs we uncovered here suggest two long-range disinhibitory circuits. In summary, we found: (1) dominant ascending inputs to the shell, (2) two subpopulations of shell excitatory neurons, and (3) two disinhibitory circuits.
Figure 1. IC excitatory and inhibitory neurons as starter cells for rabies-based monosynaptic tracing.
To reveal the neuronal organization and cell-type-specific connections in the inferior colliculus (IC), the authors used the monosynaptic rabies tracing technique combined with excitatory and inhibitory Cre driver lines to characterize the brainwide inputs to specified IC neuron types within specific subdivisions. Furthermore, combining the viral tracing with in situ hybridization (ISH) histochemistry, the authors clarified the cell types that give rise to the commissural inputs on specific cell types, allowing us to reveal parallel ascending pathways in the IC core and shell, different combinations of inputs to excitatory neurons, and two long-range disinhibitory connections in the IC.
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