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Anatomic Evidence for Information Exchange between Primary Af
PRV152-GFP was used for retrograde labeling of TG neurons innervating the IWAEC. (From BrainVTA)
The viruses used in this article are in the table below
Pseudorabies virus  PRV152-GFP
Haixia Liu, Xutao Zhu, Yun Ling, Xiaobin He, Lei Pei, Zhidan Zhang, Fang Yang and Fuqiang Xu
Pub Date: 2018-07-10,  DOI: 10.1167/iovs.18-24308, Email: [email protected]
PURPOSE. Our previous studies suggested that mechanosensitive trigeminal ganglion (TG) nerve endings innervating the inner wall of the anterior eye chamber (IWAEC) might play a role in baroreception of the IOP. It has been reported that mechanosensitive TG nerve endings also innervate the dura mater. An acute IOP elevation evokes eye pain accompanied by an ipsilateral headache, suggesting that information exchange may occur between the primary afferent neurons (PANs) innervating the IWAEC and the dura mater. To verify the information exchange between PANs of the two locations, we investigated the anatomic connection between them.
METHODS. Non–trans-synaptic tracers, 1,1 -dilinoleyl-3,3,3 ,3 -tetramethylindo-carbocyanine, 4-chlorobenzenesulfonate (FAST Dil) and cholera toxin subunit-B with a 488-nm fluorescent tag (CTB-488), were applied to the dura of the anterior cranial fossa (DACF) and the anterior eye chamber (AEC) to label the PANs. A trans-synaptic tracer, GFP-expressing pseudorabies virus (PRV152), was injected into the AEC while FAST Dil was applied to the DACF to explore the connection between PANs. Fluorescent localization in the TG was studied with a confocal fluorescent microscope.
RESULTS. Nine days after rats were treated with CTB-488 in the AEC and FAST Dil on the DACF, FAST Dil-labeled (red), and CTB-488-labeled (green) TG neurons were observed in the medial part of the TG, while double-labeled neurons were absent. If PRV152 was used to substitute CTB-488, then FAST Dil (red) and PRV152 (green) double-labeled TG neurons and axons were observed 3 days later.
CONCLUSIONS. Our results indicate that synapses exist between PANs of the IWAEC and the DACF, providing anatomic evidence for information exchange between them.

Figure 1. PRV152 crosses the synapse from a TG neuron innervating the IWAEC into another TG neuron innervating the DACF.
To verify the information exchange between PANs of the two locations, the authors investigated the anatomic connection between them. The results provide anatomic evidence for information exchange between PANs innervating the IWAEC and the DACF. It presents a valuable clue to further explore the mechanism that coordinates the regulation of the IOP and the ICP in mammals.
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