COVID-19 Coronavirus Research Tools
Pre-Made AAVs Center
Neuronal Tracing Tool


Construction and Packaging Service of virus Vector

Construction and Packaging Services of AAV

Large scale AAV production

Conditioned stimulus presentations alter anxiety level in fea
Retrograde virus hSyn-Cre in LHA and Ef1α-DIO-GCaMP6s virus in vCA1 were used to examine the activity of the LHA-projecting vCA1 neurons. (All viruses were packaged by BrainVTA)
The viruses used in this article from BrainVTA are in the table below
CRE Recombinase  PT-0136 rAAV-hSyn-Cre-WPRE-pA
Calcium sensors  PT-0071 rAAV-Ef1α-DIO-GCaMP6s-WPRE-pA
Yujie Zhang, Kunfu Ouyang, Tatiana V Lipina, Hong Wang and Qiang Zhou
Pub Date: 2019-03-29,  DOI: 10.1186/s13041-019-0445-4, Email:
It is generally believed that fear is rapidly triggered by a distinct cue while anxiety onset is less precise and not associated with a distinct cue. Although it has been claimed that both processes can be measured with certain independence of each other, it is unclear how exactly they differ. In this study, we measured anxiety in mice that received discriminative fear conditioning using behavioral, heart rate and calcium (Ca2+) responses in the ventral hippocampal CA1 (vCA1) neurons. We found that the occurrence of fear significantly interfered with anxiety measurements under various conditions. Diazepam reduced basal anxiety level but had no effect during the presentation of conditioned stimulus (CS). Injection of an inhibitory peptide of PKMzeta (ZIP) into the basolateral amygdala almost entirely abolished CS-triggered fear expression and reduced anxiety to basal level. Heart rate measures suggested a small reduction in anxiety during CS-. Calcium responses in the lateral hypothalamus-projecting vCA1 neurons showed a steady decay during CS suggesting a reduced anxiety. Thus, under our experimental conditions, CS presentations likely reduce anxiety level in the fear-conditioned mice.
Figure. 1 Calcium responses in vCA1 neurons that projecting to lateral hypothalamus during CS presentation. 
In this study, the authors asked a simple question: can CS induce measurable changes in the anxiety level in mice received auditory fear conditioning? They used various measurements of anxiety in mice, together with measurement of fear responses. They found some evidence that anxiety level is, actually, reduced during CS presentation in fear-conditioned mice.
BrainVTA offers viral vector construction & virus packaging services for AAV, LV, RABV, PRV, HSV and VSV that help researchers explore questions about genes, neurons, circuitry structure, function of brain network, mechanism and treatment of diseases.
If you have any needs, just email us at

50% discount for Pre-Made AAVs