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Whole Brain Mapping of Long-Range Direct Input to Glutamaterg
Monosynaptic RV(From BrainVTA) was used to label the whole-brain inputs to specific cell types in the Mop and MOs simultaneously.
The viruses used in this article from BrainVTA are in the table below
Tracing Helper  PT-0095 rAAV2/9-Ef1a-DIO-BFP-2a-TVA-WPRE-pA
 PT-0023 rAAV2/9-Ef1a-DIO-RG-WPRE-pA
Pan Luo, Anan Li, Yanxiao Zheng, Yutong Han, Jiaojiao Tian, Zhengchao Xu, Hui Gong and Xiangning Li
Pub Date: 2019-04-17,  DOI: 10.3389/fnana.2019.00044, Email:
Long-range neuronal circuits play an important role in motor and sensory information processing. Determining direct synaptic inputs of excited and inhibited neurons is important for understanding the circuit mechanisms involved in regulating movement. Here, we used the monosynaptic rabies tracing technique, combined with fluorescent micro-optical sectional tomography, to characterize the brain-wide input to the motor cortex (MC). The whole brain dataset showed that the main excited and inhibited neurons in the MC received inputs from similar brain regions with a quantitative difference. With 3D reconstruction we found that the distribution of input neurons, that target the primary and secondary MC, had different patterns. In the cortex, the neurons projecting to the primary MC mainly distributed in the lateral and anterior portion, while those to the secondary MC distributed in the medial and posterior portion. The input neurons in the subcortical areas also showed the topographic shift model, as in the thalamus, the neurons distributed as outer and inner shells while the neurons in the claustrum and amygdala were in the ventral and dorsal part, respectively. These results lay the anatomical foundation to understanding the organized pattern of motor circuits and the functional differences between the primary and secondary MC.

Figure. 1 Representative images of selected regions with monosynaptic inputs to the glutamatergic and GABAergic neurons in the motor cortex.
Using a monosynaptic rabies tracing strategy  (viruses are From BrainVTA), combined with continuously imaging using the fMOST system, the authors mapped the whole brain inputs to specific cell types in the subregions of the MC.
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