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Optimization of the Fluorescent Protein Expression Level Base
Retrograde trans-multisynaptic tracers PRV were used for neural circuit tracing.
The viruses used in this article are in the table below
Pseudorabies virus  PRV152
Fan Jia, Pei Lv, Huan Miao, Xiangwei Shi, Hongjun Mei, Li Li, Xiaoqin Xu, Sijue Tao and Fuqiang Xu
Pub Date: 2019-06-21,  DOI: 10.3389/fnana.2019.00063,  Email:
Mapping the neural circuits facilitates understanding the brain’s working mechanism. Pseudorabies virus (PRV; Bartha stain) as a tracer can infect neurons and retrogradely transport in neural circuits. To illuminate the network, tracers expressing reporter genes at a high level are needed. In this study, we optimized the expression level of reporter genes and constructed two new retrograde trans-multisynaptic tracers PRV531 and PRV724, which separately express more robust green and red fluorescent proteins than the existing retrograde tracers PRV152 and PRV614. PRV531 and PRV724 can be used for mapping the neural circuit of the central nervous system (CNS) and the peripheral nervous system (PNS). Overall, our work adds two valuable tracers to the toolbox for mapping neural circuits.

Figure 1. PRV531 labels the neural circuit of central nervous system (CNS) and peripheral nervous system (PNS).
Enhancing the expression level of the reporter facilitates the observation of the labeled neural circuit. Therefore, to achieve this aim, the authors have constructed a trans-multi-synaptic tracer based on the PRV Bartha strain by inserting a series of elements, which can express robust fluorescent protein and can be used for mapping the neural circuit.
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