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Tactile modulation of memory and anxiety requires dentate gra
A rabies virus (RABV)-based retrograde monosynaptic tracing system (From BrainVTA) was used to identify that TEE-tagged neuronal ensembles in DG received inputs mainly from LEC layer 2 neurons. Chemogenetics manipulation was used to selectively manipulate TEE-tagged neuronal ensembles in dDG or vDG.
The viruses used in this article from BrainVTA are in the table below
RV  R01002 RABV-EnvA-ΔG-DsRed
Tracing Helper  PT-0021 AAV2/9-Ef1α-DIO-His-EGFP-2A-TVA-WPRE-pA
 PT-0023 AAV2/9-Ef1α-DIO-RVG-WPRE-pA
CRE Recombinase  PT-0136  AAV2/1-hSyn-Cre-WPRE-pA
Pub Date: 2020-11-27,  DOI: 10.1038/s41467-020-19874-8   Email:
Chi Wang, Hui Liu, Kun Li, Zhen-Zhen Wu, Chen Wu, Jing-Ying Yu, Qian Gong, Ping Fang, Xing-Xing Wang, Shu-Min Duan, Hao Wang, Yan Gu, JiHu, Bing-Xing Pan,Mathias V. Schmidt, Yi-Jun Liu& Xiao-Dong Wang

Image resouce:L
Touch can positively influence cognition and emotion, but the underlying mechanisms remain unclear. Here, we report that tactile experience enrichment improves memory and alleviates anxiety by remodeling neurons along the dorsoventral axis of the dentate gyrus (DG) in adult mice. Tactile enrichment induces differential activation and structural modification of neurons in the dorsal and ventral DG, and increases the presynaptic input from the lateral entorhinal cortex (LEC), which is reciprocally connected with the primary somatosensory cortex (S1), to tactile experience-activated DG neurons. Chemogenetic activation of tactile experience-tagged dorsal and ventral DG neurons enhances memory and reduces anxiety respectively, whereas inactivation of these neurons or S1-innervated LEC neurons abolishes the beneficial effects of tactile enrichment. Moreover, adulthood tactile enrichment attenuates early-life stress-induced memory deficits and anxiety-related behavior. Our findings demonstrate that enriched tactile experience retunes the pathway from S1 to DG and enhances DG neuronal plasticity to modulate cognition and emotion.
Fig. 1 Transsynaptic tracing of S1-innervated LEC cells that project to TEE-tagged DG neurons.
To dissect the potentially differential roles of the dorsal DG (dDG) and the ventral DG (vDG) in tactile enrichment-evoked cognitive and emotional effects, the authors combined functional labeling of neurons and chemogenetics to manipulate tactile experience enrichment-activated neuronal ensembles (“TEE-tagged” neurons) in dDG or vDG. The results show that recurrent tactile enrichment enhanced synaptic plasticity in dDG and vDG granule cells to improve memory and reduce anxiety, respectively, and attenuated early life stress-induced behavioral and structural abnormalities.

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