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Rabies Virus Pseudotyped with CVS-N2C Glycoprotein as a Power
RV were used to compare their retrograde infection efficiencies in vivo,AAV-retro were used for retrograde labeling in the long-projection cortical and hippocampal neurons.  (All viruses were packaged by BrainVTA)
The viruses used in this article from BrainVTA are in the table below
RV  SAD-RV(DG)-B19(G)-EGFP
 SAD-RV(DG)-N2C(G)-EGFP
 SAD-RV(DG)-N2C(G)-mCherry
Control  rAAV2-retro-EF1a-EYFP
 rAAV2-retro-EF1a-mCherry
Pub Date: 2019-08-23, DOI: 10.1007/s12264-019-00423-3Email: [email protected]
Xutao Zhu • Kunzhang Lin • Qing Liu • Xinpei Yue • Huijie Mi • Xiaoping Huang • Xiaobin He • Ruiqi Wu • Danhao Zheng • Dong Wei • Liangliang Jia • Weilin Wang • Anne Manyande • Jie Wang • Zhijian Zhang • Fuqiang Xu
Efficient viral vectors for mapping and manipulating long-projection neuronal circuits are crucial in structural and functional studies of the brain. The SAD strain rabies virus with the glycoprotein gene deleted pseudotyped with the N2C glycoprotein (SAD-RV(DG)-N2C(G)) shows strong neuro-tropism in cell culture, but its in vivo efficiency for retrograde gene transduction and neuro-tropism have not been systematically characterized. We compared these features in different mouse brain regions for SAD-RV-N2C(G) and two other widely-used retrograde tracers, SAD-RV(DG)-B19(G) and rAAV2-retro. We found that SAD-RV(DG)-N2C(G) enhanced the infection efficiency of long-projecting neurons by *10 times but with very similar neuro-tropism, compared with SAD-RV(DG)-B19(G). On the other hand, SAD-RV(DG)-N2C(G) had an infection efficiency comparable with rAAV2-retro, but a more restricted diffusion range, and broader tropism to different types and regions of long-projecting neuronal populations. These results demonstrate that SAD-RV(DG)-N2C(G) can serve as an effective retrograde vector for studying neuronal circuits.

Fig1. The retrograde gene transduction efficiency of SAD-RV(DG)-N2C(G)-EGFP is higher than that of SAD-RV(DG)-B19(G)-EGFP.
To compare the in vivo retrograde gene transduction efficiency and tropisms of the RABV enveloped by N2C(G) to long-projecting neuronal circuits and the two outstanding retrograde tracers (SAD strain RABV and rAAV2-retro), the authors found that N2C(G)-enveloped SAD-RV(DG)-N2C(G) has high retrograde gene transduction efficiency and broad neuro-tropism to target the inputs to neuronal circuits by using the virus tools. (From BrainVTA)

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