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A Visual Circuit Related to the Nucleus Reuniens for the Spat
To specifically infect postsynaptic Re neurons with mCherry or hM3Dq-mCherry, AAV-Cre was injected into the vLGN/IGL. For monosynaptic tracing the inputs to Re, RV virus was injected into Re. (From BrainVTA)
The viruses used in this article from BrainVTA are in the table below
RV  R01002 RV-ENVA-ΔG-DsRed
Tracing Helper  PT-0062 AAV2/9-EF1a-DIO-EGFP-TVA
 PT-0023 rAAV2/9-Ef1α-DIO-RVG
 PT-0022 AAV2/9-hSyn-EGFP-2a-TVA-2a-RVG-WPREs-pA
CRE Recombinase  PT-0136 AAV2/1-hSyn-Cre
Optogenetic  PT-0002 AAV2/9-Ef1a-DIO-hChR2(H134R)-mCherry
Control  PT-0100 AAV2/9-hSyn-mCherry
 PT-0013 AAV2/9-Ef1a-DIO-mCherry
Pub Date: 2020-11-10,  DOI: 10.1016/j.neuron.2020.10.023  Email:
Xiaodan Huang, Pengcheng Huang, Lu Huang, Zhengfang Hu, Xianwei Liu, Jiawei Shen, Yue Xi, Yan Yang, Yunwei Fu, Qian Tao, Song Lin, Anding Xu, Fuqiang Xu, Tian Xue, Kwok-Fai So, Haohong Li and Chaoran Ren
Light exerts profound effects on cognitive functions across species, including humans. However, the neuronal mechanisms underlying the effects of light on cognitive functions are poorly understood. In this study, we show that long-term exposure to bright-light treatment promotes spatial memory through a di-synaptic visual circuit related to the nucleus reuniens (Re). Specifically, a subset of SMI-32-expressing ON-type retinal ganglion cells (RGCs) innervate CaMKIIa neurons in the thalamic ventral lateral geniculate nucleus and intergeniculate leaflet (vLGN/IGL), which in turn activate CaMKIIa neurons in the Re. Specific activation of vLGN/IGL-projecting RGCs, activation of Re-projecting vLGN/IGL neurons, or activation of postsynaptic Re neurons is sufficient to promote spatial memory. Furthermore, we demonstrate that the spatial-memory-promoting effects of light treatment are dependent on the activation of vLGN/IGL-projecting RGCs, Re-projecting vLGN/IGL neurons, and Re neurons. Our results reveal a dedicated subcortical visual circuit that mediates the spatial-memory-promoting effects of light treatment.
Fig.1 Using virus tracing systems to show the Spatial-Memory-Promoting Effects of Light Treatment
To evaluate the effect of light treatment on memory performance, combining conventional neurotracer and transneuronal virus tracing techniques (From BrainVTA), the researchers identified a di-synaptic visual circuit connecting the retina and the Re in mice. these novel results may improve our current understanding of the mechanisms underlying the effects of light on memory.

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