RV for retrograde labeling and PRV for retrograde and trans-synaptic tracing were used to systematically dissect the organization of the top–down olfactory circuits.
(All viruses were packaged by BrainVTA)
The viruses used from BrainVTA in this article are in the table below
|
RV |
R02001 RV-DG-eGFP
R02002 RV-DG-dsRed |
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PRV |
PRV531 |
Pub Date: 2019-05-08,
DOI: 10.1007/s12264-019-00385-6，
Email: [email protected]
Pengjie Wen • Xiaoping Rao • Liuying Xu • Zhijian Zhang • Fan Jia • Xiaobin He • Fuqiang Xu
Abstract Sensory processing is strongly modulated by different brain and behavioral states, and this is based on the top–down modulation. In the olfactory system, local neural circuits in the olfactory bulb (OB) are innervated by centrifugal afferents in order to regulate the processing of olfactory information in the OB under different behavioral states. The purpose of the present study was to explore the organization of neural networks in olfactory-related cortices and modulatory nuclei that give rise to direct and indirect innervations to the glomerular layer (GL) of the OB at the whole-brain scale. Injection of different recombinant attenuated neurotropic viruses into the GL showed that it received direct inputs from each layer in the OB, centrifugal inputs from the ipsilateralanterior olfactory nucleus (AON), anterior piriform cortex (Pir), and horizontal limb of diagonal band of Broca (HDB), and various indirect inputs from bilateral cortical neurons in the AON, Pir, amygdala, entorhinal cortex, hippocampus, HDB, dorsal raphe, median raphe and locus coeruleus. These results provide a circuitry basis that will help further understand the mechanism by which olfactory information-processing in the OB is regulated.
Fig1. Organization of retrogradely-labeled neurons in the OB.
To systematically understand the mechanism of top–down regulation of the different layers of the OB, the authors used monosynaptic and trans-synaptic recombinant neurotropic viral tracers separately to dissect the architecture of the top–down regulatory circuits that directly and indirectly innervate the GL of the OB. The results suggested that cortical neurons in these regions have considerable reciprocal connections and innervate the neural circuits in GL of the OB to coordinate and regulate the processing of olfactory information.
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